9LM8

Crystal structure of bvFP-Pfr21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 1
Unit cell lengths	41.625, 45.576, 59.021
Unit cell angles	102.75, 102.06, 102.21

Refinement procedure

Resolution	42.800 - 2.450
R-factor	0.2429
Rwork	0.238
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.560
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.800	2.538
High resolution limit [Å]	2.450	2.450
Rmerge	0.101
Rmeas	0.121
Number of reflections	14135	7175
<I/σ(I)>	6.6
Completeness [%]	88.2
Redundancy	3.2
CC(1/2)	0.996	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	10% v/v 2-propanol; 0.1 M sodium citrate tribasic dihydrate (pH 5.0); and 26% v/v polyethylene glycol 400