9LLU

p53 epitope specific TCR 4414A binding to p53Y220D-HLA-A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97881
Spacegroup name	I 1 2 1
Unit cell lengths	108.640, 44.050, 207.879
Unit cell angles	90.00, 104.59, 90.00

Refinement procedure

Resolution	34.770 - 2.870
R-factor	0.2665
Rwork	0.266
R-free	0.27010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.938
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.770	2.973
High resolution limit [Å]	2.870	2.870
Number of reflections	22341	2206
<I/σ(I)>	9.59
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.993	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.16M potassium citrate 0.1 M iminazole PH7.5 22% PEG 2000 10mM Magnesium formate