9LLQ
Structure of TetR2 and DNA probe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.023, 116.521, 113.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.110 - 3.100 |
| R-factor | 0.2278 |
| Rwork | 0.223 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8z6e |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.425 |
| Data reduction software | autoPX |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.689 | |
| Number of reflections | 13225 | 1251 |
| <I/σ(I)> | 23.32 | |
| Completeness [%] | 98.7 | |
| Redundancy | 11.76 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Sodium chloride,BIS-TRIS,PEG 3350 |
