9LLO

a designed heterotrimer combining natural and synthetic fragments

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-17
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	15.161, 54.681, 119.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.760 - 1.800
R-factor	0.1812
Rwork	0.179
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	14.155
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21rc1_5109: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.760	1.864
High resolution limit [Å]	1.800	1.800
Rmeas	0.140
Rpim		0.047
Number of reflections	9946	9946
<I/σ(I)>	32.51
Completeness [%]	99.5
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Ammonium citrate tribasic pH 7.0 20% w/v Polyethylene glycol 3350