9LLN
a collagen heterotrimer combining natural and synthetic fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-15 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.160, 48.662, 54.750 |
| Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
| Resolution | 27.650 - 1.720 |
| R-factor | 0.2204 |
| Rwork | 0.218 |
| R-free | 0.25990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 8.299 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.650 | 1.785 |
| High resolution limit [Å] | 1.720 | 1.723 |
| Rpim | 0.043 | 0.043 |
| Number of reflections | 17259 | 17529 |
| <I/σ(I)> | 29.4 | |
| Completeness [%] | 98.5 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Ammonium citrate tribasic pH 7.0 20% w/v Polyethylene glycol 3,350 |
