9LL2

Crystal structure of the Helicobacter pylori copper resistance determinant CrdA in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-29
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	37.597, 39.392, 145.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 1.640
R-factor	0.1909
Rwork	0.190
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.931
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.670
High resolution limit [Å]	1.640	1.640
Number of reflections	26939	1266
<I/σ(I)>	38.4	4.8
Completeness [%]	98.6	96.9
Redundancy	4.7	3.9
CC(1/2)	0.994	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.6	291	ammonium sulfate, bicine