9LKS
Crystal Structure of N-terminal flexible domain of the Shaft pilin EbpC from Enterococcus faecalis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 68.182, 68.182, 127.412 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.630 - 1.790 |
| R-factor | 0.16778 |
| Rwork | 0.165 |
| R-free | 0.21851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.427 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.640 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.046 | 0.681 |
| Rmeas | 0.051 | 0.752 |
| Rpim | 0.021 | 0.314 |
| Number of reflections | 17123 | 856 |
| <I/σ(I)> | 19.3 | 5.2 |
| Completeness [%] | 99.8 | 96.8 |
| Redundancy | 5.7 | |
| CC(1/2) | 0.990 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 100mM Sodium-citrate, 33% (w/v) PEG 6000 |
