9LKK

Crystal structure of C1ql1-gC1q hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	110.367, 110.634, 114.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.460 - 2.220
R-factor	0.1737
Rwork	0.172
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.460	2.300
High resolution limit [Å]	2.220	2.220
Number of reflections	70092	6923
<I/σ(I)>	14.48
Completeness [%]	100.0	100
Redundancy	2
CC(1/2)	0.999	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	protein solution: 50 mM Tris, pH 7.5, 500 mM NaCl; crystal solution: 0.1 M Na Cacodylate, pH 6.0, 25% MPD, and 0.05 M Ca acetate.