9LKF
Crystal structure of Guanine-II riboswitch in complex with guanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-06-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 2 2 2 |
| Unit cell lengths | 37.681, 48.382, 119.709 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.780 - 2.830 |
| R-factor | 0.1873 |
| Rwork | 0.185 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.438 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.710 | 2.980 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.108 | 0.810 |
| Rmeas | 0.111 | 0.881 |
| Rpim | 0.048 | 0.345 |
| Number of reflections | 5647 | 800 |
| <I/σ(I)> | 13.3 | 2.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 11.1 | |
| CC(1/2) | 0.998 | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.08 M Sodium chloride, 0.04 M Sodium cacodylate trihydrate pH 7.0, 30% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride |
