9LKC

Crystal structure of Guanine-II riboswitch in complex with 2'-deoxyguanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 2 2 2
Unit cell lengths	38.142, 48.850, 119.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.140 - 2.610
R-factor	0.2025
Rwork	0.201
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.203
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.750	2.750
High resolution limit [Å]	2.610	2.610
Rmerge	0.058	1.003
Rmeas	0.064	0.448
Rpim	0.026	1.100
Number of reflections	6808	780
<I/σ(I)>	21.7
Completeness [%]	92.9
Redundancy	11.1
CC(1/2)	0.999	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.08 M Sodium chloride, 0.04 M Sodium cacodylate trihydrate pH 7.0, 30% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride