9LJV

Structural insights into the polymerase catalyzed FAD-capping of hepatitis C viral RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	289.15
Detector technology	PIXEL
Collection date	2024-01-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98761
Spacegroup name	P 65
Unit cell lengths	140.493, 140.493, 92.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.990 - 2.250
Rwork	0.184
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.990	2.380
High resolution limit [Å]	2.250	2.250
Number of reflections	49586	7934
<I/σ(I)>	17.8
Completeness [%]	99.8
Redundancy	14.42	9.69
CC(1/2)	0.997	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	PEG 3350, 100 mM Bis-Tris propane/hydrochloric acid, pH 8.5, 200 mM Sodium malonate dibasic precipitant