9LJU

Structural insights into the polymerase catalyzed FAD-capping of hepatitis C viral RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	289.15
Detector technology	PIXEL
Collection date	2023-12-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	139.313, 139.313, 93.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.630 - 2.920
Rwork	0.174
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.062
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.020	2.890
High resolution limit [Å]	2.740	2.740
Number of reflections	26742	3635
<I/σ(I)>	8.8
Completeness [%]	98.7
Redundancy	10.8
CC(1/2)	0.986	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	PEG 3350, 100 mM Bis-Tris propane/hydrochloric acid, pH 8.5, 200 mM Sodium malonate dibasic precipitant