9LJT

Structural insights into the polymerase catalyzed FAD-capping of hepatitis C viral RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	289.15
Detector technology	PIXEL
Collection date	2023-12-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	139.843, 139.843, 92.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.920 - 3.460
Rwork	0.174
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.087
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.590	3.650
High resolution limit [Å]	3.460	3.460
Rpim		0.799
Number of reflections	13506	1943
<I/σ(I)>	5.3
Completeness [%]	99.6
Redundancy	17.1
CC(1/2)	0.969	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	PEG 3350, 100 mM Bis-Tris propane/hydrochloric acid, pH 8.5, 200 mM Sodium malonate dibasic precipitant