9LJR

Structural insights into the polymerase catalyzed FAD-capping of hepatitis C viral RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	289.15
Detector technology	PIXEL
Collection date	2023-12-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	138.876, 138.876, 93.227
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.440 - 2.740
Rwork	0.172
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.670	2.910
High resolution limit [Å]	2.740	2.740
Rmeas	0.192	0.925
Number of reflections	26892	4339
<I/σ(I)>	11.94	3.58
Completeness [%]	99.6
Redundancy	6.3
CC(1/2)	0.994	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	PEG 3350, 100 mM Bis-Tris propane/ hydrochloric acid, pH 8.5, 200 mM Sodium malonate dibasic precipitant.