9LJN

Crystal structure of Guanine-II riboswitch in complex with guanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.10202
Spacegroup name	P 2 2 2
Unit cell lengths	38.024, 49.112, 120.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.060 - 2.750
R-factor	0.2207
Rwork	0.217
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.146
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.160	2.890
High resolution limit [Å]	2.750	2.750
Rmerge	0.068	0.801
Rmeas	0.074	0.867
Rpim	0.029	0.331
Number of reflections	6256	896
<I/σ(I)>	17.4	2.7
Completeness [%]	99.1
Redundancy	12.1
CC(1/2)	0.998	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.08 M Sodium chloride, 0.04 M Sodium cacodylate trihydrate pH 7.0, 30% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride