9LJM
Structure of the periplasmic domain of MotS from Bacillus subtilis in 300 mM KCl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.680, 80.000, 55.880 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 55.220 - 1.900 |
| R-factor | 0.2125 |
| Rwork | 0.211 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.572 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.220 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.470 |
| Number of reflections | 27774 | 1775 |
| <I/σ(I)> | 9.7 | 1.8 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.995 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Na2HPO4/Citric acid pH4.2, 40% PEG300. |
