9LJL
Structure of the periplasmic domain of MotS from Bacillus subtilis in 300 mM NaCl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.510, 79.370, 55.930 |
| Unit cell angles | 90.00, 99.42, 90.00 |
Refinement procedure
| Resolution | 55.180 - 1.890 |
| R-factor | 0.1818 |
| Rwork | 0.179 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.677 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.180 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.097 | |
| Number of reflections | 28007 | 1841 |
| <I/σ(I)> | 7.5 | 2.3 |
| Completeness [%] | 99.3 | |
| Redundancy | 3 | |
| CC(1/2) | 0.993 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-NaOH pH7.5, 20% PEG8000, protein conc. 10 mg/mL. |
