9LIF

Repetitive domain (RP) 1 structure of aciniform spidroin from Latrodectus hesperus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	25.439, 57.314, 35.000
Unit cell angles	90.00, 96.46, 90.00

Refinement procedure

Resolution	29.730 - 1.380
R-factor	0.1585
Rwork	0.157
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.780	1.460
High resolution limit [Å]	1.380	1.380
Number of reflections	19796	2782
<I/σ(I)>	14.6
Completeness [%]	96.8
Redundancy	5.9
CC(1/2)	0.998	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M Tris8.0 28% w/v Polyethylene glycol 4000