9LI3
Crystal structure of FAD-dependent oxidoreductase from Marinomonas mediterranea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979137 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.911, 60.117, 95.503 |
| Unit cell angles | 90.00, 95.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.210 |
| R-factor | 0.1695 |
| Rwork | 0.168 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.977 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.820 | 2.270 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 40674 | 2971 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.3 | |
| Redundancy | 10.4 | 7.9 |
| CC(1/2) | 0.982 | 0.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20 % PEG 3350,0.2M Ammonium citrate tribasic pH 7.0 |
