9LHS

Crystal structure of an ice-binding protein from Candidatus Cryosericum odellii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	66.810, 66.810, 142.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.259 - 1.830
Rwork	0.177
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.669
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.259	1.870
High resolution limit [Å]	1.830	1.830
Rmerge	0.060	0.630
Number of reflections	29297	1774
<I/σ(I)>	35.2
Completeness [%]	100.0
Redundancy	25.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3.5 M sodium formate pH 7.0