Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.608, 73.118, 48.172 |
| Unit cell angles | 90.00, 100.52, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.000 |
| R-factor | 0.1854 |
| Rwork | 0.181 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ifj |
| RMSD bond angle | 14.650 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.880 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 16958 | 1305 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 94.9 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.995 | 0.983 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG3350,0.1M potassium chloride |
