9LFX

Streptococcus mutans FtsZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9798
Spacegroup name	P 43 21 2
Unit cell lengths	173.119, 173.119, 172.282
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.560 - 3.490
R-factor	0.225
Rwork	0.222
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.341
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.560	3.620
High resolution limit [Å]	3.490	3.490
Number of reflections	30537	30537
<I/σ(I)>	10.67
Completeness [%]	90.1
Redundancy	9.6
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	296	PEG10000, Potassium sodium tartrate, MES