9LFJ
Crystal structure of UgpB in complex with G3P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.450, 115.080, 46.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.010 - 1.340 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.19480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.866 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.010 | 1.370 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 83693 | 3856 |
| <I/σ(I)> | 25.4 | 1.8 |
| Completeness [%] | 93.0 | 59.2 |
| Redundancy | 10.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M MIB buffer (pH 7.0) and 25% (w/v) PEG 1500 |
