9LFB
Crystal structure of GpxB in complex with G3P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-15 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 136.509, 136.509, 136.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.750 - 2.000 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.19370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.750 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.380 |
| Number of reflections | 28413 | 2824 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | calcium acetate, sodium acetate (pH 4.5) and PEG 4000 |
