9LF9
Crystal structure of GpxB in complex with G1P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 136.280, 136.280, 136.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.470 - 1.680 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.16920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.894 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.470 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.110 | 1.220 |
| Number of reflections | 47905 | 3543 |
| <I/σ(I)> | 22 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 31.6 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | potassium phosphate, polyethylene glycol (PEG) 8000 and glycerol |
