9LED

Rice FBA1 in complex with fructose bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97861
Spacegroup name	I 1 2 1
Unit cell lengths	74.137, 102.384, 92.980
Unit cell angles	90.00, 93.11, 90.00

Refinement procedure

Resolution	46.420 - 2.200
R-factor	0.1908
Rwork	0.190
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.757
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.420	2.230
High resolution limit [Å]	2.200	2.200
Rmerge	0.039	0.487
Rpim	0.017	0.242
Number of reflections	64329	1788
<I/σ(I)>	30.2
Completeness [%]	92.8
Redundancy	6.5
CC(1/2)	1.000	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	PEG 6000