Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 58.450, 58.450, 222.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.150 - 1.940 |
| R-factor | 0.2206 |
| Rwork | 0.218 |
| R-free | 0.26520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.199 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.230 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.059 | 1.273 |
| Rmeas | 0.060 | 1.308 |
| Rpim | 0.292 | |
| Number of reflections | 17731 | 1233 |
| <I/σ(I)> | 33 | 2.6 |
| Completeness [%] | 99.8 | |
| Redundancy | 30.2 | 19.6 |
| CC(1/2) | 1.000 | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | 200 mM Ammonium sulfate 20% w/v PEG 3350 pH 6.0 |
