9LBY
Crystal Structure of Maize Lipoxygenase3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.510, 75.040, 107.720 |
| Unit cell angles | 98.91, 101.88, 113.71 |
Refinement procedure
| Resolution | 27.360 - 2.080 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.24210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.360 | 9.300 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 109557 | 10884 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 97.0 | |
| Redundancy | 6 | |
| CC(1/2) | 0.993 | 0.636 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.1 M BIS-TRIS pH5.5,25% w/v Polyethylene glycol 3,000 |
