9LBT
DPPIV-VAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-07 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.827, 126.370, 136.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.410 - 1.990 |
| R-factor | 0.1691 |
| Rwork | 0.169 |
| R-free | 0.19540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 6.862 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.410 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rpim | 0.037 | 0.037 |
| Number of reflections | 138319 | 138311 |
| <I/σ(I)> | 14.93 | |
| Completeness [%] | 98.7 | |
| Redundancy | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.25 M Ammonium acetate, 0.1 M Tris pH 8.5, 21% w/v Polyethylene glycol 3350 |
