9LBF
The crystal structure of the truncated PAK2 containing D368N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 167.325, 167.325, 212.863 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.890 - 2.620 |
| Rwork | 0.198 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.614 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.710 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.159 | 1.245 |
| Number of reflections | 34739 | 3443 |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 11 |
| CC(1/2) | 0.983 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M DL-malic acid pH5.51, 1.4M (NH4)2SO4, 0.025% CH2Cl2 |
