9LA6

Crystal structure of anti-CRISPR protein AcrIE7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-11-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	62.330, 61.997, 87.260
Unit cell angles	90.00, 102.36, 90.00

Refinement procedure

Resolution	42.620 - 2.680
R-factor	0.2647
Rwork	0.265
R-free	0.33420
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.634
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.620	2.779
High resolution limit [Å]	2.680	2.683
Number of reflections	17657	916
<I/σ(I)>	18.49
Completeness [%]	98.7
Redundancy	1.9
CC(1/2)	0.999	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	PEG 8000, HEPES/Sodium hydroxide