9L9X

Structure of SPARTA in complex with guide DNA and a 20nt target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	140.420, 140.420, 176.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.500 - 6.700
R-factor	0.2505
Rwork	0.247
R-free	0.31900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.525
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.270	6.880
High resolution limit [Å]	6.700	6.700
Rpim	0.048	0.900
Number of reflections	3669	277
<I/σ(I)>	13.7
Completeness [%]	98.8	100
Redundancy	17.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M BICINE, pH=8.5, 4.6% PEG4000