9L8G

R115A mutant of Ferredoxin-NADP+ reductase from maize root - Oxidized form, low X-ray dose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.90000
Spacegroup name	P 32 2 1
Unit cell lengths	59.170, 59.170, 186.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.410 - 1.350
R-factor	0.1733
Rwork	0.173
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h59
RMSD bond length	0.007
RMSD bond angle	0.984
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.410	1.370
High resolution limit [Å]	1.350	1.350
Number of reflections	83861	4086
<I/σ(I)>	8.9	1.5
Completeness [%]	99.8
Redundancy	5.7
CC(1/2)	0.997	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293.15	PEG2000 29.5%