9L8D

Structure of frog TREX1-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-08-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97849
Spacegroup name	I 41 2 2
Unit cell lengths	144.684, 144.684, 82.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.170 - 2.030
R-factor	0.2034
Rwork	0.202
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tqn
RMSD bond length	0.003
RMSD bond angle	0.638
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.170	2.100
High resolution limit [Å]	2.030	2.030
Rpim	0.019	0.174
Number of reflections	28440	2788
<I/σ(I)>	16.9	3.35
Completeness [%]	99.9	99.25
Redundancy	31.9	32.5
CC(1/2)	0.981	0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Citric acid pH 4.0, 20% PEG 6000