9L8B
Structure of fish TREX2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-12-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.470, 78.510, 66.920 |
| Unit cell angles | 90.00, 103.07, 90.00 |
Refinement procedure
| Resolution | 32.220 - 1.990 |
| R-factor | 0.2324 |
| Rwork | 0.231 |
| R-free | 0.26460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.477 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rpim | 0.044 | 0.717 |
| Number of reflections | 23028 | 2818 |
| <I/σ(I)> | 10.5 | 1.1 |
| Completeness [%] | 99.3 | 97.4 |
| Redundancy | 11.5 | 7.9 |
| CC(1/2) | 0.999 | 0.368 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M MgCl2, 0.1 M Tris-HCl pH8.5, 35% PEG 4000 |
