9L87
Structure of fish TREX1-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-12-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 54.598, 135.960, 193.969 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.710 - 1.990 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.21370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.145 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.130 | 2.030 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rpim | 0.025 | 0.342 |
| Number of reflections | 50061 | 4896 |
| <I/σ(I)> | 12.1 | 1.94 |
| Completeness [%] | 99.5 | 98.95 |
| Redundancy | 24.6 | 20.2 |
| CC(1/2) | 0.999 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.3 M C6H5Na3O7, 20% PEG 3350 |
