9L85
Structure of fish TREX1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-12-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.680, 86.860, 132.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.350 - 2.910 |
| R-factor | 0.2372 |
| Rwork | 0.234 |
| R-free | 0.26930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.533 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.550 | 3.060 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rpim | 0.086 | 0.622 |
| Number of reflections | 19553 | 2723 |
| <I/σ(I)> | 8.7 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.8 | 13.3 |
| CC(1/2) | 0.997 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.3 M KSCN, 15% PEG 3350 |
