9L6Z

Crystal structure of the L7Ae derivative protein LS12 in RNA-free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	33.800, 54.270, 54.866
Unit cell angles	90.00, 93.97, 90.00

Refinement procedure

Resolution	38.540 - 1.800
R-factor	0.1732
Rwork	0.171
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bw0
RMSD bond length	0.007
RMSD bond angle	0.953
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.800	1.800
Rmeas	0.133	0.419
Number of reflections	35224	5019
<I/σ(I)>	15.86
Completeness [%]	97.5
Redundancy	3.2	2.2
CC(1/2)	0.991	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG 2,000 MME