9L56

Plastid-localized exonuclease 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	300
Detector technology	PIXEL
Collection date	2024-05-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 31 2 1
Unit cell lengths	107.147, 107.147, 132.433
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.180 - 2.420
R-factor	0.2314
Rwork	0.230
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.717
Data scaling software	XDS (1.0.5)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.790	2.550
High resolution limit [Å]	2.420	2.420
Rmerge	0.129	1.600
Rmeas	0.133
Rpim	0.029	0.800
Number of reflections	33874	4877
<I/σ(I)>	15.4
Completeness [%]	99.9	99.6
Redundancy	10.5	10.6
CC(1/2)	0.999	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298.15	0.2 M ammonium sulfate, 0.1M Tris-HCl pH 7.5, 20% w/v PEG 4000 and soaked with 10 mM MnCl2