9L48

Crystal structure of HLA-C*12:02-RV9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	95
Detector technology	CCD
Collection date	2023-05-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97849
Spacegroup name	P 21 21 21
Unit cell lengths	52.170, 71.860, 124.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.140 - 1.900
R-factor	0.1762
Rwork	0.175
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.815
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.140	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.106	0.477
Rmeas	0.116	0.522
Rpim	0.046	0.209
Number of reflections	37484	2355
<I/σ(I)>	14.8
Completeness [%]	99.2	98.3
Redundancy	11	10.7
CC(1/2)	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2 M sodium citrate tribasic dihydrate, 20% w/v Polyethylene glycol 3,350