9L3J
Crystal structure of endo-processive xyloglucanase Xeg5A from Aspergillus oryzae with GXG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.295, 106.913, 83.742 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 38.980 - 2.740 |
| R-factor | 0.2399 |
| Rwork | 0.237 |
| R-free | 0.28930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.804 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | XDS (Jan 10, 2022) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.060 | 2.840 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.142 | 1.013 |
| Rmeas | 0.165 | |
| Rpim | 0.084 | 0.603 |
| Number of reflections | 36896 | 3367 |
| <I/σ(I)> | 6.7 | 0.9 |
| Completeness [%] | 98.7 | 90.4 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.996 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, 20% PEG4000, 10% 2-propanol |
