Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.347, 79.347, 92.827 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.210 - 1.490 |
| R-factor | 0.1442 |
| Rwork | 0.143 |
| R-free | 0.16540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.203 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.230 | 1.570 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.861 | |
| Number of reflections | 55336 | 8119 |
| <I/σ(I)> | 17.1 | 2.8 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 18 | 12.9 |
| CC(1/2) | 0.998 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 288 | 2.0 M Ammonium sulfate, 0.1 M Tris(hydroxymethyl)aminomethane-HCl, pH 8.5 |
