9L1I

Crystal structure of PpRib7 in complex with NADPH (P212121 form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-06
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.217, 79.439, 139.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.750 - 2.080
R-factor	0.2011
Rwork	0.199
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.094	0.740
Number of reflections	32360	3194
<I/σ(I)>	18.2
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.1 M BIS-TRIS pH 6.5 45% v/v Polypropylene glycol P 400