9L1F
Crystal structure of PpRib7 in complex NADPH (P1 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.057, 50.609, 63.246 |
| Unit cell angles | 87.66, 86.43, 64.73 |
Refinement procedure
| Resolution | 26.170 - 1.600 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.053 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.033 | 0.280 |
| Number of reflections | 61353 | 11385 |
| <I/σ(I)> | 23.7 | |
| Completeness [%] | 96.1 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 15% (w/v) polypropylene glycol 3350, and 0.1 M magnesium formate dehydrate |
