9L0N
The crystal structure of the beta-glucosidase Ks5A7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2024-11-14 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.037, 112.936, 99.389 |
| Unit cell angles | 90.00, 101.23, 90.00 |
Refinement procedure
| Resolution | 27.340 - 2.500 |
| R-factor | 0.2177 |
| Rwork | 0.216 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.559 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.340 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.190 | 0.533 |
| Number of reflections | 68878 | 10068 |
| <I/σ(I)> | 8.1 | 3.6 |
| Completeness [%] | 99.8 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.976 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 15% PEG 3350, 200 mM NaCl, 100 mM Tris-HCl pH 8.0, 100 mM CaCl2. |
