9L08
Cyclic Trimer of Helix-Linked Cytochrome c555
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.704, 104.519, 73.705 |
| Unit cell angles | 90.00, 92.95, 90.00 |
Refinement procedure
| Resolution | 46.120 - 1.600 |
| R-factor | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.804 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 112336 | 17912 |
| <I/σ(I)> | 17.9 | 2 |
| Completeness [%] | 98.4 | 97.5 |
| Redundancy | 6.4 | 6 |
| CC(1/2) | 0.998 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200 mM lithium sulfate, 20% (w/v) PEG 8000, 200 mM sodium acetate buffer, pH 4.5 |
