9KZ5
Crystal structure of TapT-MTA complex from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.660, 42.830, 76.980 |
| Unit cell angles | 90.00, 96.79, 90.00 |
Refinement procedure
| Resolution | 22.180 - 2.350 |
| R-factor | 0.2243 |
| Rwork | 0.222 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.539 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (V1.21.2-5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.180 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.245 | 1.245 |
| Number of reflections | 16335 | 1191 |
| <I/σ(I)> | 6.5 | 1.9 |
| Completeness [%] | 99.6 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M Lithium sulfate, 0.1M Bis-Tris propane pH 7.0,27% w/v PEG 3350 |
