9KYT
Crystal structure of TapT-SAM complex from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-04 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.848, 42.251, 75.492 |
| Unit cell angles | 90.00, 97.18, 90.00 |
Refinement procedure
| Resolution | 37.450 - 2.050 |
| R-factor | 0.2005 |
| Rwork | 0.199 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.922 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (V1.21.2-5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.390 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.063 | 0.460 |
| Number of reflections | 23123 | 3436 |
| <I/σ(I)> | 7.3 | 2.5 |
| Completeness [%] | 97.9 | 99.8 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Lithium sulfate monohydrate, 0.1 M Tris hydrochloride pH 8.5, 30 % w/v polyethylene glycol 4000 |
