9KYS

the Ca2+/CaM-CASK-ARD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	37.662, 76.786, 130.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.730 - 1.760
R-factor	0.1992
Rwork	0.197
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cdl
RMSD bond length	0.007
RMSD bond angle	0.880
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.760	1.760
Number of reflections	37870	3659
<I/σ(I)>	3.9
Completeness [%]	98.4	96.8
Redundancy	10.7	10.6
CC(1/2)	0.998	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	magnesium chloride, PEG 3350