9KYL
Crystal structure of human TRIP4 in complex with 11bp dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.339, 74.798, 73.304 |
| Unit cell angles | 90.00, 102.61, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.150 |
| R-factor | 0.2167 |
| Rwork | 0.214 |
| R-free | 0.26280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.170 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5156) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.139 | 0.458 |
| Rmeas | 0.154 | 0.516 |
| Rpim | 0.065 | 0.232 |
| Number of reflections | 21150 | 2072 |
| <I/σ(I)> | 3 | 1.8 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 5.3 | 4.7 |
| CC(1/2) | 0.960 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M L-Proline, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 3,350 |
